IP 625: Automotive fuels and fat and oil derivates – Determination of saturated monoglycerides content in Fatty Acid Methyl Esters (FAME) – Method by GC-FID

Overview:

This document specifies a method to determine the saturated monoglyceride content in Fatty Acid Methyl Esters (FAME). This method only identifies and quantifies the following saturated monoglycerides: 1-C16:0, 2-C16:0 and 1-C18:0. The total saturated monoglyceride content is calculated by the summation of the contents of these three saturated monoglycerides. The precision has been established for FAMEs having saturated monoglycerides in the (200 to 1 500) mg/kg range.

This method is not suitable for FAME produced from or containing coconut and palm kernel oil derivatives because of overlapping of various peaks.

NOTE This Standard determines only three saturated monoglycerides, i.e. 1-C16:0, 2-C16:0 and 1-C18:0. FAMEs can contain also other saturated monoglycerides such as 1-C17:0, but these are generally much lower than the three targeted saturated monoglycerides and are therefore not included in the Standard’s scope.